期刊
NATURE METHODS
卷 10, 期 4, 页码 332-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2378
关键词
-
资金
- US National Institutes of Health (NIH) [R01 GM080148]
- NIH [5T32HG002760, 5T32GM007215-37]
- Searle Scholars Award and Shaw Scientist award
- National Science Foundation
We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据