期刊
NATURE METHODS
卷 10, 期 11, 页码 1116-1121出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2681
关键词
-
资金
- US National Institutes of Health NHGRI [P50 HG005550]
- US Department of Energy [DE-FG02-02ER63445]
- Wyss Institute for Biologically Inspired Engineering
The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas acquired immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but it can mediate only a single activity at a time within any given cell. Here we characterize a set of fully orthogonal Cas9 proteins and demonstrate their ability to mediate simultaneous and independently targeted gene regulation and editing in bacteria and in human cells. We find that Cas9 orthologs display consistent patterns in their recognition of target sequences, and we identify an unexpectedly versatile Cas9 protein from Neisseria meningitidis. We provide a basal set of orthogonal RNA-guided proteins for controlling biological systems and establish a general methodology for characterizing additional proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据