期刊
NATURE METHODS
卷 10, 期 8, 页码 737-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2556
关键词
-
资金
- Swedish Research Council
- German Research Foundation (DFG)-Center of Nanoscale Microscopy and Molecular Physiology of the Brain
- German Ministry of Research (BMBF)
- Korber Foundation, Hamburg, through the European Science Prize
- Medical Research Council [MC_UU_12010/9, MR/K01577X/1] Funding Source: researchfish
- MRC [MR/K01577X/1, MC_UU_12010/9] Funding Source: UKRI
We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 mm x 100 mm-sized fields of view in <1 s using 116,000 such doughnuts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据