期刊
NATURE METHODS
卷 10, 期 10, 页码 973-+出版社
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2600
关键词
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资金
- US National Institutes of Health (NIH) [DP20D008586]
- National Science Foundation (NSF) Faculty Early Career Development (CAREER) Award [CBET-1151035]
- NIH [R03AR061042, U54HG004563, EB015300, HL109442, R01AR48852, T32GM008555]
- American Heart Association Scientist Development Grant [10SDG3060033]
- NSF
- American Heart Association
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1151035] Funding Source: National Science Foundation
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
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