期刊
NATURE METHODS
卷 10, 期 12, 页码 1206-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2691
关键词
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资金
- Wellcome Trust [060208/Z/00/Z]
- WT [093305/Z/10/Z]
- Medical Research Council
- European Research Council
- Science Foundation Ireland [07/IN.1/B1836, 12/IA/1255]
- US National Institutes of Health [GM75915, P50GM073210, U54GM094599]
- Biotechnology and Biological Sciences Research Council
- Cambridge Overseas Trust
- Taiwan Ministry of Education
- Royal Society
- Biotechnology and Biological Sciences Research Council [BB/K01983X/1, BB/G011915/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J01835X/1] Funding Source: researchfish
- Medical Research Council [G1000819] Funding Source: researchfish
- BBSRC [BB/G011915/1, BB/K01983X/1] Funding Source: UKRI
- EPSRC [EP/J01835X/1] Funding Source: UKRI
- MRC [G1000819] Funding Source: UKRI
- Wellcome Trust [093305/Z/10/Z] Funding Source: Wellcome Trust
We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.
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