期刊
NATURE METHODS
卷 10, 期 10, 页码 1028-+出版社
NATURE PORTFOLIO
DOI: 10.1038/NMETH.2641
关键词
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资金
- US National Institutes of Health (NIH) Office of Research Infrastructure Programs [P40 0D010440]
- NIH [T32 CA009156, R01 GM085309, CA20535, R01 GM083071]
- Howard Hughes postdoctoral fellowship from the Helen Hay Whitney Foundation
- Canadian Institutes of Health Research [234765]
- US National Science Foundation (NSF) [MCB 1157767]
- NSF [IOS 0917726]
- Direct For Biological Sciences [1157767] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1157767] Funding Source: National Science Foundation
Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility of model organisms. Here we report a method to edit the C. elegans genome using the clustered, regularly interspersed, short palindromic repeats (CRISPR) RNA-guided Cas9 nuclease and homologous recombination. We demonstrate that Cas9 is able to induce DNA double-strand breaks with specificity for targeted sites and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, we generated gfp knock-ins and targeted mutations. Together our results outline a flexible methodology to produce essentially any desired modification in the C. elegans genome quickly and at low cost. This technology is an important addition to the array of genetic techniques already available in this experimentally tractable model organism.
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