期刊
NATURE METHODS
卷 10, 期 1, 页码 60-U80出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2277
关键词
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资金
- Jane Coffin Childs postdoctoral fellowship
- Fulbright fellowship
- Agence Nationale pour la Recherche [ANR-08-PCVI-0013]
- US National Institutes of Health [GM31627]
- HHMI
- Center for Cancer Research, National Cancer Institute
- Agence Nationale de la Recherche (ANR) [ANR-08-PCVI-0013] Funding Source: Agence Nationale de la Recherche (ANR)
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
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