期刊
NATURE METHODS
卷 9, 期 6, 页码 594-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2017
关键词
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资金
- Austrian Science Foundation [FWF P21712, APW01220FW]
- European Commission
- Austrian Science Fund (FWF) [P 21712] Funding Source: researchfish
- Austrian Science Fund (FWF) [P21712] Funding Source: Austrian Science Fund (FWF)
We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.
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