期刊
NATURE METHODS
卷 9, 期 8, 页码 815-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2098
关键词
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资金
- Agence Nationale de la Recherche
- Fondation Louis D de l'Institut de France
- Ville de Paris
- Inserm Avenir
- Ecole des Neurosciences de Paris
We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
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