期刊
NATURE METHODS
卷 9, 期 4, 页码 360-U59出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1894
关键词
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资金
- Bundesministerium fur Bildung und Forschung [GO-Bio (0315980)]
- EU [SyBoSS 242129]
- Deutsche Forschungsgemeinschaft [SPP1356 BU1400/3-1, SFB655]
Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.
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