期刊
NATURE METHODS
卷 9, 期 12, 页码 1198-U96出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.2213
关键词
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资金
- Max Planck Society
- Deutsche Forschungsgemeinschaft (DFG)
The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.
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