期刊
NATURE METHODS
卷 9, 期 3, 页码 273-U77出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1857
关键词
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资金
- US National Eye Institute [R01EY017925, R21EY020985]
- Medical University of South Carolina
We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.
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