期刊
NATURE METHODS
卷 9, 期 2, 页码 145-151出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1828
关键词
-
资金
- Biotechnology and Biological Sciences Research Council, UK
Bisulfite conversion of genomic DNA combined with next-generation sequencing (BS-seq) is widely used to measure the methylation state of a whole genome, the methylome, at single-base resolution. However, analysis of BS-seq data still poses a considerable challenge. Here we summarize the challenges of BS-seq mapping as they apply to both base and color-space data. We also explore the effect of sequencing error; and contaminants on inferred methylation levels and recommend the most appropriate way to analyze this type of data.
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