期刊
NATURE METHODS
卷 9, 期 6, 页码 575-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1972
关键词
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资金
- Federal Ministry of Education and Research [01GN1009B, 01GN1008C, 01GN1008A]
- German Research Foundation [SFB-TR3 D2]
- European Union [FP7-HEALTH-2007-B-22943-NeuroStemcell, HEALTH-F5-2010-266753-SCRTox]
- Stem Cell Network North Rhine Westphalia [L-072.0058]
- Else Kroner-Fresenius-Stiftung Fellowship
- Hertie Foundation
Forced expression of proneural transcription factors has been shown to direct neuronal conversion of fibroblasts. Because neurons are postmitotic, conversion efficiencies are an important parameter for this process. We present a minimalist approach combining two-factor neuronal programming with small molecule-based inhibition of glycogen synthase kinase-3 beta and SMAD signaling, which converts postnatal human fibroblasts into functional neuron-like cells with yields up to >200% and neuronal purities up to >80%.
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