期刊
NATURE METHODS
卷 9, 期 9, 页码 913-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.2137
关键词
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资金
- Helen Hay Whitney Foundation
- US National Institutes of Health-National Cancer Institute [1R21CA160080]
We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.
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