期刊
NATURE METHODS
卷 9, 期 5, 页码 467-U76出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1930
关键词
-
资金
- Australian National Health and Medical Research Council [566640]
- Miegunyah Trust
We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据