期刊
NATURE METHODS
卷 8, 期 10, 页码 871-U147出版社
NATURE PORTFOLIO
DOI: 10.1038/NMETH.1694
关键词
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资金
- US National Science Foundation
- Stanford University
- Human Frontier Science Program
- Machiah Foundation
- Lawrence Livermore National Laboratory
- Office of Naval Research
- US National Institutes of Health Nanomedicine Development Center for Optical Control of Biological Function
- National Science Foundation Center for Biophotonics
- Packard
- Bill and Melinda Gates Foundation
- Paul G. Allen Family Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1063292] Funding Source: National Science Foundation
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior. Here we introduce a miniature (1.9 g) integrated fluorescence microscope made from mass-producible parts, including a semiconductor light source and sensor. This device enables high-speed cellular imaging across similar to 0.5 mm(2) areas in active mice. This capability allowed concurrent tracking of Ca2+ spiking in >200 Purkinje neurons across nine cerebellar microzones. During mouse locomotion, individual microzones exhibited large-scale, synchronized Ca2+ spiking. This is a mesoscopic neural dynamic missed by prior techniques for studying the brain at other length scales. Overall, the integrated microscope is a potentially transformative technology that permits distribution to many animals and enables diverse usages, such as portable diagnostics or microscope arrays for large-scale screens.
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