期刊
NATURE METHODS
卷 8, 期 7, 页码 581-U93出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1614
关键词
-
资金
- Canadian Institutes of Health Research [MOP-93571, RMF-82499, MSH-95337]
- Natural Sciences and Engineering Research Council of Canada [RGPIN 312140]
- Michael Smith Foundation for Health Research
Heterogeneity in cell populations poses a major obstacle to understanding complex biological processes. Here we present a microfluidic platform containing thousands of nanoliter-scale chambers suitable for live-cell imaging studies of clonal cultures of nonadherent cells with precise control of the conditions, capabilities for in situ immunostaining and recovery of viable cells. We show that this platform mimics conventional cultures in reproducing the responses of various types of primitive mouse hematopoietic cells with retention of their functional properties, as demonstrated by subsequent in vitro and in vivo (transplantation) assays of recovered cells. The automated medium exchange of this system made it possible to define when Steel factor stimulation is first required by adult hematopoietic stem cells in vitro as the point of exit from quiescence. This technology will offer many new avenues to interrogate otherwise inaccessible mechanisms governing mammalian cell growth and fate decisions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据