期刊
NATURE METHODS
卷 8, 期 11, 页码 937-940出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1714
关键词
-
资金
- US National Institutes of Health [HG3456, GM67945]
Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.
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