期刊
NATURE METHODS
卷 9, 期 1, 页码 68-U178出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1774
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资金
- Tri-Institutional Training Program in Chemical Biology
- Tri-Institutional Training Program in Computational Biology and Medicine
- Irma T. Hirschl/Monique Weill-Caulier Trust
- Lieber Center for Schizophrenia Research and Treatment at Columbia University
Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
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