期刊
NATURE METHODS
卷 8, 期 12, 页码 1056-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1775
关键词
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资金
- Damon Runyon Cancer Research Foundation [DRG-1937-07]
- US National Institutes of Health [P01 NS055923, R01 NS058502, R01HL081186]
- Helmsley Stem Cell Starter
The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNADNADNA-binding sites of transcription factors involved in motor neuron specification.
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