期刊
NATURE METHODS
卷 9, 期 1, 页码 81-U197出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1802
关键词
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资金
- European Research Council [EU/232814-StemCeLLMark]
- National Research Foundation of Korea [NRF-2011-357-C00093]
- The Centre van Biomedical Genetic
- The Dutch Cancer Society [KWF/Hubr2007-3956]
- EU [Tornado-KBBE-222720, EU/236954-ICSC LGR5, Ti Pharma/T3-106, KNAW/3V]
- EMBO
he study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.
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