期刊
NATURE METHODS
卷 8, 期 11, 页码 941-943出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1733
关键词
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资金
- National Research Foundation of Korea [2011-0000402, 2011-0013568, 2011-27177, 2011-0019357, 2011-0028267]
- National Research Foundation of Korea [2011-0013568, 2011-0019357] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry.
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