期刊
NATURE METHODS
卷 8, 期 8, 页码 691-U129出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1649
关键词
-
资金
- US National Institute of Neurological Disorders and Stroke [NS 04842]
The protein ubiquitin is an important post-translational modifier that regulates a wide variety of biological processes. In cells, ubiquitin is apportioned among distinct pools, which include a variety of free and conjugated species. Although maintenance of a dynamic and complex equilibrium among ubiquitin pools is crucial for cell survival, the tools necessary to quantify each cellular ubiquitin pool have been limited. We have developed a quantitative mass spectrometry approach to measure cellular concentrations of ubiquitin species using isotope-labeled protein standards and applied it to characterize ubiquitin pools in cells and tissues. Our method is convenient, adaptable and should be a valuable tool to facilitate our understanding of this important signaling molecule.
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