期刊
NATURE METHODS
卷 8, 期 12, 页码 1037-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1740
关键词
-
资金
- Australian Stem Cell Centre
- National Health and Medical Research Council of Australia [606586, 573707]
- National Heart Foundation (Australia) [G 08M 3711, CR 08S 3958]
- Netherlands Organization for Scientific Research
- Netherlands Institute of Regenerative Medicine
- Victorian State Government
- National Health and Medical Research Council of Australia's Independent Research Institutes
- Marie Curie Co-fund Action
- National Health and Medical Research Council of Australia
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据
分享论文
