期刊
NATURE METHODS
卷 8, 期 12, 页码 1041-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1770
关键词
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资金
- University of Washington's Proteomics Resource [UWPR95794]
- Thermo Scientific Pierce Human In vitro Translation Research
- US National Institutes of Health [P41RR011823, U54HG004592]
We report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using in vitro-synthesized proteins. We demonstrate the approach using human transcription factors, which are typically difficult, low-abundance targets and empirically derived proteotypic peptides for 98% of the target proteins. We show that targeted proteomic assays developed using our approach facilitate robust in vivo quantification of human transcription factors.
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