期刊
NATURE METHODS
卷 7, 期 5, 页码 377-U59出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1447
关键词
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资金
- European Union [FP7-HEALTH-F4-2008-201418]
- US National Institutes of Health [GM33289]
- Human Frontier Science Program [GP0054/2009-C]
- Damon Runyon Cancer Research Foundation [DRG-1997-08]
We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula for its precision exaggerates beyond Fisher's information limit, and weighted least-squares may do worse, whereas maximum-likelihood fitting is practically optimal.
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