期刊
NATURE METHODS
卷 7, 期 2, 页码 133-U69出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1414
关键词
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资金
- Alexander von Humboldt Foundation
- Massachusetts Life Science Center
- Pew Charitable Trusts
- US National Institutes of Health [R01HG004401, U54HG03067, U01ES017155]
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.
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