期刊
NATURE METHODS
卷 7, 期 12, 页码 985-U116出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1533
关键词
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资金
- Human Frontier Science Program [RGP0053/2005-C]
- German Federal Ministry of Education and Research [05KS4BY1/7]
- US National Institutes of Health
- National Institute of Arthritis and Musculoskeletal and Skin Diseases, and the National Cancer Institute, Center for Cancer Research [HHSN26120080001E]
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at similar to 36-nm (Rayleigh) and similar to 70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.
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