期刊
NATURE METHODS
卷 7, 期 2, 页码 130-U63出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1417
关键词
-
资金
- Wellcome Trust [WT079643]
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
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