期刊
NATURE METHODS
卷 7, 期 6, 页码 451-U57出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1454
关键词
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资金
- Lundbeck Foundation
- Howard Hughes Medical Institute
- McKnight Grant
- Genome Canada
- Genome British Columbia
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0950955] Funding Source: National Science Foundation
We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
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