期刊
NATURE METHODS
卷 7, 期 6, 页码 473-U87出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1460
关键词
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资金
- Academy of Finland [113485, 118450]
- International Human Frontier Science Program Organization
- Biocentrum Helsinki
- Sigrid Juselius Foundation
- Magnus Ehrnrooth Foundation
- University of Helsinki
- Helsinki Biomedical Graduate School
- Academy of Finland (AKA) [113485, 118450, 113485, 118450] Funding Source: Academy of Finland (AKA)
The ubiquitin-proteasome system (UPS) orchestrates many cellular and tissue-specific processes by degrading damaged and key regulatory proteins. To enable investigation of UPS activity in different cell types in a living animal, we developed a photoconvertible fluorescent UPS reporter system for live imaging and quantification of protein degradation in Caenorhabditis elegans. Our reporter consists of the photoconvertible fluorescent protein Dendra2 targeted for proteasomal degradation by fusion to the UbG76V mutant form of ubiquitin. In contrast to previous reporters, this system permits quantification of UPS activity independently of protein synthesis. Our reporter revealed that UPS-mediated protein degradation varies in a cell type-specific and age-dependent manner in C. elegans.
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