期刊
NATURE METHODS
卷 7, 期 11, 页码 913-U67出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1516
关键词
-
资金
- Cancer Genomics Centre
- European Heads of Research Councils
- European Science Foundation
- EU
Targeted genomic enrichment followed by next-generation DNA sequencing has dramatically increased efficiency of mutation-discovery efforts. We describe a protocol for genomic enrichment of pooled barcoded samples in a single assay that increases experimental flexibility and efficiency. We screened 770 genes (1.4 megabases) in thirty N-ethyl-N-nitrosourea (ENU)-mutagenized rats and identified known variants at >96% sensitivity as well as new mutations at a false positive rate <1 in 8 megabases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据