期刊
NATURE METHODS
卷 6, 期 6, 页码 443-U62出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1330
关键词
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资金
- Netherlands Organization for Scientific Research (NWO)
- Leukemia and Lymphoma Society
- US National Institutes of Health [K99/R00, R01 GM80783]
- National Institute of General Medical Sciences [GM080853]
- Sandler New Technologies
- Fight For Mike foundation
Short hairpin RNA libraries are limited by low efficacy of many shRNAs and by off-target effects, which give rise to false negatives and false positives, respectively. Here we present a strategy for rapidly creating expanded shRNA pools (similar to 30 shRNAs per gene) that are analyzed by deep sequencing (EXPAND). This approach enables identification of multiple effective target-specific shRNAs from a complex pool, allowing a rigorous statistical evaluation of true hits.
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