期刊
NATURE METHODS
卷 6, 期 3, 页码 215-218出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1300
关键词
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资金
- Dreyfus Foundation Teacher-Scholar Award program
- US National Science Foundation (CAREER) [CHE 449952]
- Alzheimer's Association (IIRG) [08-91256]
- National Institutes of Health R01 program [AG028490]
- Estonian Science foundation programs
We present an approach that accelerates protein solid-state NMR 5-20-fold using paramagnetic doping to condense datacollection time (to similar to 0.2 s per scan), overcoming a long-standing limitation on slow recycling owing to intrinsic H-1 T-1 longitudinal spin relaxation. Using low-power schemes under magic-angle spinning at 40 kHz, we obtained two-dimensional C-13-C-13 and C-13-N-15 solid-state NMR spectra for several to tens of nanomoles of beta-amyloid fibrils and ubiquitin in 1-2 d.
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