期刊
NATURE METHODS
卷 6, 期 6, 页码 458-U86出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1327
关键词
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资金
- Medical Research Council
- Harvard Materials Research Science and Engineering Center
- Wellcome Trust
- Human Frontier Science
- Biotechnology and Biological Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/F012845/1] Funding Source: researchfish
- Medical Research Council [G0400709] Funding Source: researchfish
- BBSRC [BB/F012845/1] Funding Source: UKRI
- MRC [G0400709] Funding Source: UKRI
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [820484] Funding Source: National Science Foundation
The dynamic reshaping of tissues during morphogenesis results from a combination of individual cell behaviors and collective cell rearrangements. However, a comprehensive framework to unambiguously measure and link cell behavior to tissue morphogenesis is lacking. Here we introduce such a kinematic framework, bridging cell and tissue behaviors at an intermediate, mesoscopic, level of cell clusters or domains. By measuring domain deformation in terms of the relative motion of cell positions and the evolution of their shapes, we characterized the basic invariant quantities that measure fundamental classes of cell behavior, namely tensorial rates of cell shape change and cell intercalation. In doing so we introduce an explicit definition of cell intercalation as a continuous process. We mapped strain rates spatiotemporally in three models of tissue morphogenesis, gaining insight into morphogenetic mechanisms. Our quantitative approach has broad relevance for the precise characterization and comparison of morphogenetic phenotypes.
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