期刊
NATURE METHODS
卷 6, 期 7, 页码 520-U79出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1345
关键词
-
资金
- Danish Cancer Society [DP06031]
- Carlsberg Foundation [2005-1-641]
- Landsteiner Foundation of Blood Transfusion [0522]
- Melanoma Research Alliance
- Dutch Cancer Society [UL 2007-3825]
The use of fluorescently labeled major histocompatibility complex multimers has become an essential technique for analyzing disease- and therapy-induced T-cell immunity. Whereas classical major histocompatibility complex multimer analyses are well-suited for the detection of immune responses to a few epitopes, limitations on human-subject sample size preclude a comprehensive analysis of T-cell immunity. To address this issue, we developed a combinatorial encoding strategy that allows the parallel detection of a multitude of different T-cell populations in a single sample. Detection of T cells from peripheral blood by combinatorial encoding is as efficient as detection with conventionally labeled multimers but results in a substantially increased sensitivity and, most notably, allows comprehensive screens to be performed. We obtained proof of principle for the feasibility of large-scale screening of human material by analysis of human leukocyte antigen A3- restricted T-cell responses to known and potential melanoma-associated antigens in peripheral blood from individuals with melanoma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据