期刊
NATURE METHODS
卷 6, 期 8, 页码 585-U49出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1347
关键词
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资金
- Biotechnology and Biological Sciences Research Council [BB/F008333/1] Funding Source: Medline
- Medical Research Council Funding Source: Medline
- Wellcome Trust [088150] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/F008333/1] Funding Source: researchfish
- BBSRC [BB/F008333/1] Funding Source: UKRI
We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex to multiple collisions, we released the intact complex largely devoid of detergent. This enabled us to characterize both subunit stoichiometry and lipid binding in 4 membrane protein complexes.
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