期刊
NATURE METHODS
卷 7, 期 1, 页码 50-U9出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1406
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资金
- US National Science Foundation
- US National Institutes of Health [R01AR054396]
- California Institute for Regenerative Medicine [RN2-00919]
- Burroughs Wellcome Fund
- Packard Foundation
- Leona M. and Harry B. Helmsley Charitable Trust
- Sandler Family Supporting Foundation
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR054396] Funding Source: NIH RePORTER
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
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