期刊
NATURE METHODS
卷 6, 期 5, 页码 351-353出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1317
关键词
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资金
- Scientific Research on Advanced Medical Technology of the Ministry of Labor, Health and Welfare of Japan
- Japanese Ministry of Education, Science and Technology.
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer ( FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca2+ concentration and caspase-3 activation in the same apoptotic cells.
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