期刊
NATURE METHODS
卷 5, 期 11, 页码 955-957出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.1264
关键词
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资金
- US National Institutes of Health [R01 EB008087, T32 GM007183, R01 HL56416, R01 HL67384, P01 HL53586]
A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
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