期刊
NATURE METHODS
卷 6, 期 1, 页码 91-98出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1281
关键词
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资金
- W. M. Keck Foundation
- Ellison Foundation
- Dana-Farber Cancer Institute Strategic Initiative awarded
- US National Institutes of Health [5P50HG004233, 2R01HG001715]
- University of Ghent [GOA12051401]
- Fonds Wetenschappelijk Onderzoek-Vlanderen [G. 0031.06]
- FWO-V awarded
- Genome Canada
- Ontario Genomics Institute awarded
- Fonds de la Recherche Scientifique
- [R01 ES015728]
- [5U54CA112952]
- [5U01CA105423]
- [R01 HG003224]
- [F32 HG004098]
Information on protein-protein interactions is of central importance for many areas of biomedical research. At present no method exists to systematically and experimentally assess the quality of individual interactions reported in interaction mapping experiments. To provide a standardized confidence-scoring method that can be applied to tens of thousands of protein interactions, we have developed an interaction tool kit consisting of four complementary, high-throughput protein interaction assays. We benchmarked these assays against positive and random reference sets consisting of well documented pairs of interacting human proteins and randomly chosen protein pairs, respectively. A logistic regression model was trained using the data from these reference sets to combine the assay outputs and calculate the probability that any newly identified interaction pair is a true biophysical interaction once it has been tested in the tool kit. This general approach will allow a systematic and empirical assignment of confidence scores to all individual protein-protein interactions in interactome networks.
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