期刊
NATURE METHODS
卷 5, 期 2, 页码 135-146出版社
NATURE PORTFOLIO
DOI: 10.1038/nmeth.f.202
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资金
- Medical Research Council [G0400717] Funding Source: Medline
- NIGMS NIH HHS [U54 GM074942, P50 GM062412, GM074942, U54 GM074898, U54 GM074958, U54 GM074942-02, GM62412, U54 GM074945] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Medical Research Council [G0400717] Funding Source: researchfish
- MRC [G0400717] Funding Source: UKRI
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
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