期刊
NATURE METHODS
卷 5, 期 4, 页码 311-313出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1196
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资金
- NIGMS NIH HHS [GM42516, R01 GM042516] Funding Source: Medline
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP-proliferating cell nuclear antigen (PCNA).
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