4.8 Article

In vivo immunogold labeling confirms large-scale chromatin folding motifs

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NATURE METHODS
卷 5, 期 4, 页码 311-313

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NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1196

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  1. NIGMS NIH HHS [GM42516, R01 GM042516] Funding Source: Medline

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The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP-proliferating cell nuclear antigen (PCNA).

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