期刊
NATURE METHODS
卷 6, 期 1, 页码 79-81出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1278
关键词
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资金
- Japan Science and Technology Agency, JST
- REIMEI Research Resources of Japan Atomic Energy Agency
- The Sumitomo Foundation
- Ministry of Education, Culture, Sports, Science and Technology of Japan
We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.
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