期刊
NATURE MEDICINE
卷 17, 期 10, 页码 1225-1227出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm.2470
关键词
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资金
- Spanish Ministry of Science and Innovation (MICINN)
- European Research Council (ERC)
- Instituto de Salud Carlos III [RD09/0076/00036]
- Pla Director d'Oncologia de Catalunya (XBTC)
- EU
- Botin Foundation
- Lilly Foundation
- Korber European Science Award
- Marie Curie Fellowship (FP7)
- Grants-in-Aid for Scientific Research [23890204] Funding Source: KAKEN
- ICREA Funding Source: Custom
Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.
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