期刊
NATURE MEDICINE
卷 17, 期 2, 页码 175-178出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm.2294
关键词
-
资金
- Global Centers of Excellence Program [F12]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20390287]
- Ministry of Health, Labor and Welfare of Japan
- Grants-in-Aid for Scientific Research [20390287] Funding Source: KAKEN
- Austrian Science Fund (FWF) [F12] Funding Source: Austrian Science Fund (FWF)
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrPSc) has generated the potential for sensitive detection of prions. Here we developed a new PrPSc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of >= 1 fg of PrPSc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据