期刊
NATURE MEDICINE
卷 16, 期 12, 页码 1444-U130出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm.2260
关键词
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资金
- US National Institutes of Health [EY008061, EY009339, EY019880, EY019031, EY020715, P30 EY11373]
- State of Ohio Department of Development and Third Frontier Commission [TECH 09-004]
- European Life Scientist Organization
- Klaus Tschira Foundation
Multiphoton excitation fluorescence microscopy (MPM) can image certain molecular processes in vivo. In the eye, fluorescent retinyl esters in subcellular structures called retinosomes mediate regeneration of the visual chromophore, 11-cis-retinal, by the visual cycle. But harmful fluorescent condensation products of retinoids also occur in the retina. We report that in wild-type mice, excitation with a wavelength of similar to 730 nm identified retinosomes in the retinal pigment epithelium, and excitation with a wavelength of similar to 910 nm revealed at least one additional retinal fluorophore. The latter fluorescence was absent in eyes of genetically modified mice lacking a functional visual cycle, but accentuated in eyes of older wild-type mice and mice with defective clearance of all-trans-retinal, an intermediate in the visual cycle. MPM, a noninvasive imaging modality that facilitates concurrent monitoring of retinosomes along with potentially harmful products in aging eyes, has the potential to detect early molecular changes due to age-related macular degeneration and other defects in retinoid metabolism.
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