期刊
NATURE MEDICINE
卷 16, 期 1, 页码 123-U164出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm.2071
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资金
- US National Institutes of Health-National Institute of Allergy and Infectious Diseases [F32AI063846]
- Wilhelmina Research Fund [OZF-02-004]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [F32AI063846] Funding Source: NIH RePORTER
The B cell lymphoma-6 (Bcl-6) and Bcl-xL proteins are expressed in germinal center B cells and enable them to endure the proliferative and mutagenic environment of the germinal center. By introducing these genes into peripheral blood memory B cells and culturing these cells with two factors produced by follicular helper T cells, CD40 ligand (CD40L) and interleukin-21 (IL-21), we convert them to highly proliferating, cell surface B cell receptor (BCR)-positive, immunoglobulin-secreting B cells with features of germinal center B cells, including expression of activation-induced cytidine deaminase (AID). We generated cloned lines of B cells specific for respiratory syncytial virus and used these cells as a source of antibodies that effectively neutralized this virus in vivo. This method provides a new tool to study B cell biology and signal transduction through antigen-specific B cell receptors and for the rapid generation of high-affinity human monoclonal antibodies. (C) 2010 Nature America, Inc. All rights reserved.
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