期刊
NATURE MEDICINE
卷 14, 期 4, 页码 454-458出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm1692
关键词
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资金
- NCI NIH HHS [U54 CA136429-01, U54 CA136429, U54 CA105296] Funding Source: Medline
- NIDDK NIH HHS [P30 DK056339, K08 DK067618-06, R03 DK075603-03, P30 DK56339, K08 DK067618, R03 DK075603] Funding Source: Medline
A combination of targeted probes and new imaging technologies provides a powerful set of tools with the potential to improve the early detection of cancer. To develop a probe for detecting colon cancer, we screened phage display peptide libraries against fresh human colonic adenomas for high- affinity ligands with preferential binding to premalignant tissue. We identified a specific heptapeptide sequence, VRPMPLQ, which we synthesized, conjugated with fluorescein and tested in patients undergoing colonoscopy. We imaged topically administered peptide using a fluorescence confocal microendoscope delivered through the instrument channel of a standard colonoscope. In vivo images were acquired at 12 frames per second with 50-mu m working distance and 2.5-mu m ( transverse) and 20-mu m ( axial) resolution. The fluorescein- conjugated peptide bound more strongly to dysplastic colonocytes than to adjacent normal cells with 81% sensitivity and 82% specificity. This methodology represents a promising diagnostic imaging approach for the early detection of colorectal cancer and potentially of other epithelial malignancies.
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